Preliminary Phytochemical Evaluation and HPTLC Fingerprinting of Leaves of Azadirachta indica
نویسندگان
چکیده
The fingerprint profiles of methanol extract and ethyl acetate sub fraction of Azadirachta indica leaves were conducted using preliminary phytochemical screening and high performance thin layer chromatography (HPTLC) analysis. Preliminary phytochemical screening was performed using various biochemical tests. Alkaloid, flavonoid, tannin, saponin and phenol were present in both the extract/subfraction. Toluene:Ethyl Acetate:Formic acid::5:4:1 was the finalized solvent system for both the extract and sub fraction indicating it to be the best solvent system for determination of phytochemicals of the studied plant. HPTLC finger print of methanol extract showed 16 peaks and the Rf values ranged in between 0.03 to 0.96. Similarly, ethyl acetate sub fraction confirmed 18 peaks and the Rf values ranged in between 0.03 to 0.95. HPTLC fingerprint analysis of A. indica (leaf) can be used for the exact detection of the plant. Also it is useful as a phytochemical marker and a technique to check the genetic variability in various plant species.
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